Enzyme Kineticsalkaline phosphatase
[pNPP] 1.0 mMpH 8.025 °Cno inhibitor
A405 (live)0.000pNP yellow · 1 cm path
raterun the assay to measure
M–M checkv = Vmax·[S]÷(Km+[S]) → 0.0320
Km(app)0.50 mMVmax 0.100 µmol/min at 37 °C, pH 8
v rises with [S] then plateaus at Vmax — the active sites are all busy
Ready — set [pNPP], pH, temperature; then run the assay.
Biochemistry Lab 03 · Enzyme Kinetics — drag to orbit, scroll to zoom